The Bernardine church and monastery in Lviv is located in the city's Old Town, south of the market square. The monastery along with the Roman Catholic church of St. Andrew, today the Greek Catholic church of St. Andrew, now belong the Order of St. Basil the Great.

The Franciscan Observantists, known in the region as Bernardines after their monastery in Kraków in order to distinguish them from Franciscan Conventuals, were invited to Lviv by Andrzej Odrowąż in 1460. In 1509 the monastery was plundered by the Moldovan hospodar Bogdan III cel Orb. In the 17th century the present day church and monastery were constructed in the same place in the style of Italian and Dutch mannerism and consecrated in 1630. As the complex was located outside Lviv's city walls it was equipped with its own fortifications from the east and south, mostly taken apart at the beginning of the 19th century. In 1733 square belfry was added to the complex and in 1736 a monument to Saint John of Dukla, who died in the monastery in 1484, was built in front of the church.